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KMID : 0545120080180050918
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 5 p.918 ~ p.925
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High Cell Density Culture of Anabaena variabilis with Controlled Light Intensity and Nutrient Supply
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Yoon Jong-Hyun
Shin Jong-Hwan
Ahn Eun-kyung
Park Tai-Hyun
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Abstract
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Controlling the light energy and major nutrients is important for high cell density culture of cyanobacterial cells. The growth phase of Anabaena variabilis can be divided into an exponential growth phase and a deceleration phase. In this study, the cell growth in the deceleration phase showed a linear growth pattern. Both the period of the exponential growth phase and the average cell growth rate in the deceleration phase increased by controlling the light intensity. To control the light intensity, the specific irradiation rate was maintained above 10 mmol/s/g dry cell by increasing the incident light intensity stepwise. The final cell density increased by controlling the nutrient supply. For the control of the nutrient supply, nitrate, phosphate, and sulfate were intermittently added based on the growth yield, along with the combined control of light intensity and nutrient concentration. Under these control conditions, both final cell concentration and cell productivity increased, to 8.2 g/l and 1.9 g/l/day, respectively.
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KEYWORD
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Cyanobacteria, high cell density culture, specific irradiation rate, nutrient supply, growth yield
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